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| Registros recuperados : 29 | |
| 2. |  | ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; ASTÚA, J. F.; MACHADO, M. A.; KITAJIMA, E. W. Estudo da variabilidade genética do vírus da pinta verde do maracujazeiro através de SSCP (Single Strand Conformational Polymorphism). In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25., 2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. Resumo I - Virologia (Divisão P).| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | PEREIRA, J. A.; ANTONIOLO-LUIZON, R.; LOCALI-FABRIS, E. C.; KUBO, K. S.; MACHADO, M. A.; FREITAS-ÁSTUA, J. Estudo da variabilidade genética da possível capa protéica do Citrus leprosis virus virus C (CiLV-C) em espécies de citros de 14 estados brasileiros. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 928.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 7. | | KITAJIMA, E. W.; CALEGARIO, R. F.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; BASTIANEL, M.; FREITAS-ASTÚA, J.; FRANCISCHINI, F. In situ detection of CiLV-C in the mite vector Brevipalpus phoenicis, evidence for a circulative type of virus/vector relationship and a model for the virus circulation in the mite. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 8. | | KITAJIMA, E. W.; CALEGARIO, R. F.; NOVELLI, V. M.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; FRANCISCHINI, F.; FREITAS-ASTUA, J. In situ detection and immunolocalization of the Citrus leprosis virus cytoplasmic type (CiLV) in the mite vector and evidences that the virus/vector relationship is of circulative type. In: INTERNATIONAL SCIENTIFIC SEMINAR ON PLANT HEALTH, 6.; LATIN AMERICAN AND CARIBBEAN SYMPOSIUM, 2., 2008, La Habana, Cuba. Acarina Biodiversity: their use, protection and conservation. Havana, [s.n.], 2008. 1 CD ROM| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 9. | | PEREIRA, J. A.; ANTONIOLI - LUIZON, R.; NICOLINI, F.; LOCALI-FABRIS, E. C.; KUBO, K. S.; M ACHADO, M. A.; FREITAS-ASTÚA, J. Análise da variabilidade genética do gene da capa protéica do vírus da leprose dos citros. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., p. 287, ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 10. | | ASTUA, J. de F.; KITAJIMA, E. W.; KUBO, K. S.; BASTIANEL, M.; CRISTOFANI-VALY, M.; LOCALI-FABRIS, E.; NOVELLI, V. M.; MACHADO, M. A. The atypical Brevipalpus-transmitted viruses and their interactions with host plants and mite vectors. In: EMBO WORLD LECTURE COURSE VIRUS-HOST: PARTNERS IN PATHOGENICITY, 2010, San Jose. [Abstracts...].San Jose: EMBO, 2010. p. 40. Organizado por Cecilia Ramirez.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 11. | | KUBO, K. S.; STUART, R. M.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; LOCALI-FABRIS, E. C.; COLETTA-FILHO, H. D.; MACHADO, M. A.; KITAJIMA, E. W. Evaluation of the genetic variability of orchid fleck virus by single-strand conformational polymorphism analysis and nucleotide sequencing of a fragment from the nucleocapsid. Archives of Virology, v. 154, n. 6, p. 1009-1014, mai. 2009.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 12. | | LOCALI-FABRIS, E. C.; MAFRA, V. S.; MARTINS, P. K.; RIBEIRO- ALVES, M.; FRANCISCO, C. S.; FREITAS ASTUA, J. de; KISHI, L. T.; MACHADO, M. A. Gene set enrichment analysis in sweet orange plants infected by Candidatus Liberibacter americanus. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 0058 PSO
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 13. | | FREITAS-ÁSTUA, J.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; NOVELLI, V. M.; SILVA-PINHATI, A. C.; BASÍLIO-PALMIERI, A. C.; TARGON, M. L. P. N.; MACHADO, M. A. Differentially expressed stress-related genes in the compatible citrus-Citrus leprosis virus interaction. Genetics and Molecular Biology, Ribeirão Preto, v. 30, n. 3, p. 980-990, 2007.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 14. | | NOVELLI, V. M.; ASTUA, J. de F.; LOCALI-FABRIS, E. C.; BASTIANEL, M.; ANTONIOLI-LUIZON, R.; RIBEIRO, B. M.; MACHADO, M. A. Detecção do vírus da leprose dos citros no ácaro vetor. Laranja, Cordeiropólis, v. 28, n. 1/2, 2007 Disponível em: Acesso em: 26 out. 2010.
Pdf: 04_313 desenvolvimento [1]. pdf
Secção: Fitopalogia| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 15. | | COSTA, F. M.; FREITAS-ASTUA, J.; BASTIANEL, M.; LOCALI-FABRIS, E.; MACHADO, M. A.; KUBO, K. S.; GONDIM JÚNIOR, M. G. C.; NOVELLI, V. M.; PEREIRA, J. A. Detecção do vírus da mancha anelar do cafeeiro (CoRSV) nas diferentes fases do ácaro vetor Brevipalpus phoenicis. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 919.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 16. | | KUBO, K. S.; NOVELLI, V. M.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; ANTONIOLI-LUIZON, R.; MACHADO, M. A.; FREITAS-ASTUA, J. Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR. Experimental Applied Acarology, Dordrechet, v. 54, n. 1, jan. 2011.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 17. | | FERREIRA, P. de T. O.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J.; ANTONIOLI-LUIZON, R.; GOMES, R. T.; MACHADO, M. A.; KITAJIMA, E. W. Caracterização de um vírus baciliforme isolado de Solanum violaefolium transmitido pelos ácaros Brevipalpus phoenicis e Brevipalpus obovatus (Acari: Tenuipalpidae). Summa Phytopathologica, Botucatu, v. 33, n. 3, p. 264-269, July/Sept. 2007.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Mandioca e Fruticultura. |
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| 18. | | NICOLINI, F.; BASTIANEL, M.; SCHONS, J.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; PEREIRA, J. A.; KUBO, K. S.; NOVELLI, V. M.; MACHADO, M. A.; FREITAS-ASTÚA, J. Comparação entre os títulos de vírus transmitidos por ácaros Brevipalpus por RT-qPCR. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 19. | | CALEGARIO, R. F.; KITAJIMA, E. W.; LOCALI-FABRIS, E. C.; SALAROLI, R. B.; FREITAS-ASTÚA, J.; STACH- MACHADO, D. R.; CASERTA, R.; MACHADO, M. A. In situ immunocytochemistry of citrus leaves infected with Citrus leprosis virus cytoplasmic type (CiLV-C). Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 20. | | NOVELLI, V. M.; FREITAS-ASTUA, J.; RIBEIRO, B. M.; LOCALI-FABRIS, E. C.; NAVIA, D.; KITAJIMA, E. W.; MACHADO, M. A. Prevalence of the Cardinium symbiont and genetic variability of Brevipalpus phoenicis (Acari: Tenuipalpidae) populations from Brazil. In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 181-182. Abstract 335| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 29 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
03/05/2010 |
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Data da última atualização: |
02/08/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OCHOA-CORONA, F. M.; LOCALI-FABRIS, E. C.; FREITAS-ASTÚA, J. |
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Afiliação: |
Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF. |
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Título: |
An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement |
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Idioma: |
Inglês |
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Notas: |
Abstracts of presentations, Annual Meeting of the APS 2009. p. S28. |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. |
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Thesagro: |
Brevipalpus Phoenicis; Fruta Cítrica. |
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Thesaurus NAL: |
Citrus. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/748515/1/An-RT-PCR-procedure-for-detection-and-surveillance-of-Citrus-leprosis-virus-C-CiLV-C-in-post-entry-quarantine-stocks-of-citrus.pdf
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Marc: |
LEADER 01966nam a2200181 a 4500
001 1748515
005 2023-08-02
008 2009 bl uuuu u00u1 u #d
100 1 $aOCHOA-CORONA, F. M.
245 $aAn RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus.$h[electronic resource]
260 $aPhytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement$c2009
500 $aAbstracts of presentations, Annual Meeting of the APS 2009. p. S28.
520 $aCitrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine.
650 $aCitrus
650 $aBrevipalpus Phoenicis
650 $aFruta Cítrica
700 1 $aLOCALI-FABRIS, E. C.
700 1 $aFREITAS-ASTÚA, J.
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